This is an NIH Pathway to Independence Award, which provides an opportunity for promising postdoctoral scientists to receive both mentored and independent research support from the same award. For many disorders, including autism, the circuits and cells underlying the disease are unknown. The researcher will use a powerful genetic approach, called Translating Ribosome Affinity Purification (TRAP), to identify the complete suite of genes being employed by cells isolated from autism patients. In previous studies using autism patient cells, the TRAP methodology uncovered specific mutations in genes involved in the serotonin pathway. The current proposal seeks to confirm these findings in independent samples, and then to recapitulate this genetic mutation in a mouse to test for features of autism. Even if patients have a variety of different underlying genetic causes for a disease, one common cellular mechanism could provide a valuable target for treatment strategies. The approach proposed here may have the potential to provide those cellular targets for treatments.