Project Detail
Interagency Autism Coordinating Committee (IACC) logo
Office of Autism Research Coordination (OARC) logo

Cell-based genomic analysis in mouse models of Rett syndrome  

Rett syndrome, caused by mutations in the X-linked gene encoding methyl-CpG-binding protein 2 (MeCP2), is among the best characterized of the autism spectrum disorders. However, the biological function of MeCP2 and its pathogenic mechanisms remain unclear. Studies have implicated MeCP2 in transcription and gene expression, but further study of MeCP2-mediated gene regulation is complicated by the number of different cell types in the mammalian brain. This research utilizes a nucleic acid immunoprecipitation technique that will facilitate isolation of molecules integral to the transcription process, namely messenger RNA (mRNA) and micro RNA (miRNA). To determine gene expression profiles in distinct cell types, these 2 kinds of RNA will be isolated from glutamatergic and GABAergic neurons in the motor and cognitive areas of the mouse brain at 3 stages of postnatal development. Alterations in the gene expression profiles will be compared between wild type and MeCP2 mutant mice. Simultaneous analysis of mRNA and miRNA profiles will generate a comprehensive molecular portrait of the relevant cell types and their developmental trajectories, which can then be used to better understand Rett syndrome and the interaction of gene networks and neural networks. Project Status
ONGOING

2010

Funder National Institutes of Health
Fiscal Year Funding $513,667.00
Current Award Period 2009-2011
Project Number 5RC1MH088661-02
Principal Investigator Huang, Z. Josh
Received ARRA Funding? Yes
Strategic Plan Question Question 2: How Can I Understand What Is Happening? (Biology)
Subcategory Molecular Pathways
Strategic Plan Objective Green dot: Objective has greater than or equal to the recommended funding. 2SD. Launch three studies that target improved understanding of the underlying biological pathways of genetic conditions related to autism (e.g. Fragile X, Rett syndrome, tuberous sclerosis complex) and how these conditions inform risk assessment and individualized intervention by 2012. IACC Recommended Budget: $9,000,000 over 5 years.
Federal or Private? Federal
Institution Cold Spring Harbor Laboratory
State/Country New York
Web Link 1 Cell-based genomic analysis in mouse models of Rett syndrome (External web link)
Web Link 2 No URL available.
Web Link 3 No URL available.
New! History/Related Projects Cell-based genomic analysis in mouse models of Rett syndrome | $498,790.00 | 2009 | 1RC1MH088661-01