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Interagency Autism Coordinating Committee (IACC)
Autism Research Database
Office of Autism Research Coordination (OARC)
 
Project Element Element Description

Project Title

Project Title

Sleep Disordered Breathing, Microparticles and Proinflammation in ASD

Principal Investigator

Principal Investigator

O'Hara, Ruth

Description

Description

In a previously funded SFARI study of 100 children with autism spectrum disorder (ASD), Ruth O’Hara and her colleagues found that sleep-disordered breathing (SDB) — such as sleep apnea — occurs in more than 40 percent of children with ASD, a far higher rate than that seen in typically developing children. SDB is strongly linked to cognitive and behavioral deficits and elicits a systemic inflammatory response. Recent studies have focused on the role of microparticles (MPs) in SDB disorders. Cell-derived MPs are microvesicles of 0.05 to 1 micrometers, released through exocytic budding of the plasma membrane, following stimulation of different cell types. Characterization of MPs provides a fine-grained and quantitative measure of cell-to-cell communication. Case-control and experimental studies indicate that platelet-, endothelium- and leukocyte-derived MP levels are increased in SDB and induce increased expression of proinflammatory molecules. Individuals with ASD are well documented to exhibit elevated levels of proinflammatory cytokines and chemokines compared with controls. Although MPs have not yet been examined in ASD, these alterations have all been associated with an abnormal production of MPs in proinflammatory disorders such as SDB. O’Hara and her team aim to assess whether proinflammatory processes in ASD are at least partially relayed through SDB-mediated MP. Pilot data from O’Hara and her colleagues has shown increased numbers of endothelium MPs in children with ASD and SDB but not in children with ASD and no SDB, nor in typically developing children. However, due to their small size, detailed characterization of MPs has previously been hampered by limited detection techniques, such as flow cytometry. O’Hara’s team will utilize the latest developments in the field, nanoscale flow cytometry and nanoparticle tracking analysis, which allow analysis of particles smaller than 200 nanometers, outside the range of commonly used flow cytometry instruments. These approaches, already being implemented by the O’Hara team, will be used to examine whether MPs in individuals with ASD with SDB differ from those of individuals with ASD and no SDB, and from controls without SDB.

Funder

Funder

Simons Foundation

Fiscal Year Funding

Fiscal Year Funding

60000

Current Award Period

Current Award Period

2015-2016

Strategic Plan Question

Strategic Plan Question

Question 2: How Can I Understand What Is Happening?

Strategic Plan Objective

Strategic Plan Objective

Green dot: Objective has greater than or equal to the recommended funding. 2SE. Launch three studies that target the underlying biological mechanisms of co-occurring conditions with autism, including seizures/epilepsy, sleep disorders, wandering/elopement behavior, and familial autoimmune disorders, by 2012. IACC Recommended Budget: $9,000,000 over 5 years.

Project Link

Project Link

Sleep Disordered Breathing, Microparticles and Proinflammation in ASD (External web link)

Institution

Institution

Stanford University

State/Country

State/Country

California

Project Number

Project Number

385640

Federal or Private?

Federal or Private?

Private

Received ARRA Funding?

Received ARRA Funding?

No

History/Related Projects

History/Related Projects

N/A

 
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