It has been suggested that activation of pregnant mother's immune system by infections can affect the brain of the developing fetus and thus behavior of young and adult offspring. Most recently, it was reported in mice that increased interluekin-6 (IL-6; a chemical messenger of the immune system) in the mother's blood plays a key role in altering fetal brain development and impairing behaviors in social interactions in the offspring. Interestingly, these effects could be avoided by blocking IL-6 chemically with an antibody or by genetically altering mice so they are deficient in IL-6. Preliminary studies showed that natural citrus molecules, luteolin and diosmin, inhibit the signaling mechanism (JAK2/STAT3) in neurons which carries IL-6's signal to the nucleus. When luteolin and diosmin were applied to pregnant mice, researchers found a reduction in inflammatory markers in the brains of newborn mice. In this study, researchers will examine whether or not JAK2/STAT3 signal pathway activation could be specifically involved in brain structural abnormalities in IL-6-induced activation of the maternal immune system (presumably resulting in development of abnormal behavior in adult offspring that mimics features of autism). In addition, they will test diosmin's potential effect on improving abnormal, autistic like behaviors of offspring of IL-6 immune activated mothers. These studies could lay the foundation for diosmin as a prenatal supplement in at-risk mothers to avoid autism development in their children, much like folate is currently in use to avoid neural tube defects.